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2、2020.04.03 ...
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1. 1,2 ,4,5 gDNA prep
- check con.c by nanodrop
- loading 3ug gDNA
Discussion: nanodrop con.c not match to gel running results ( it is not a big problem)
2. 6,7,8 gDNA prep
- nanodrop con.c is little low 200 ~ 400ng/ul
Discussion: it is hard to load 3ug gDNA to check integrity
# 7.8 very hard to 3ug, try to load 1ug 2 ug
PCR:
Dilution all samples to 50ng/ul by TDW.
Normally gDNA con.c should be ~ 1 ug/ul.
If con.c is too low, directly use original gDNA
95 1min , 55 1min, 72 min x 26 cycle
Template : 100ng , actual 150ng
# 6.7.8 do gDNA prep again
IGFR -166, PIR
IGFR -166, 729
PIR, IGFR 729
IGFR 146, IGFR 474